PHARMACOLOGICAL ACTIVITIES:
kaempferol,Anti-inflammation, Anti-bacteria, Restrain cough Antioxidant，
Anti-spasmodic or Spasmolytic，AntiulcerCholeretic，Diuretic，Relieve coughing
The vacuoles of lower epidermal strips from Vicia faba exhibit an intrinsic
green fluorescence when incubated in alkaline buffers. Using an alkaline-induced
absorbance change as a spectrophotometric assay, the major pigment responsible
for this fluorescence was isolated and identified as the flavonoid: kaempferol
3-O-galactoside, 7-O-rhamnoside. The aqueous absorption maxima were 394 and 341
nanometers at pH 10.0 and 6.0, respectively, with a pKa of 8.3 and the
fluorescence emission maximum was 494 nanometers at pH 10.0. The in vivo
concentration was estimated to be between 3 and 10 micromolar. The absorption
spectrum of this flavonoid is different from the action spectrum for stomatal
opening indicating that this compound is not the photoreceptor pigment for the
blue light response of Vicia faba guard cell
